anti cited1 rabbit polyclonal antibody  (Proteintech)

Journal: Animal Bioscience

Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

doi: 10.5713/ab.24.0640

Figure Lengend Snippet: CXCL12 regulated the expression of genes and proteins related to litter size and ovarian functions. The mRNA expression level of TAF4B , CITED1 , WNT2 , WNT10B and HSD17B1 were detected after overexpression (A) and knockdown (B) of CXCL12 in GCs. (C) The protein expression levels of CITED1 and WNT10B were detected after overexpression and knockdown of CXCL12 in GCs (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, granulosa cells.

Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

Techniques: Expressing, Over Expression, Knockdown

Journal: EMBO Reports

Article Title: Differential contribution of TFE3 isoforms to cell motility and invasion

doi: 10.1038/s44319-025-00659-3

Figure Lengend Snippet: ( A ) Heatmap of differentially expressed genes in ARPE19 cells infected with either adenovirus expressing recombinant TFE3-L-Myc or TFE3-S-Myc for 30 h. ( B ) Relative quantitative RT-PCR analysis of the mRNA expression of the indicated genes in ARPE19 cells infected with either adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc, or Control (Null) for 30 h. Data are presented as mean ± SD of three independent experiments. ACP5 ( ***(a) P = 0.0001; (ns) not significant (b) P = 0.7598; ****(c) P < 0.0001; ***(d) P = 0.0003; (ns) not significant (e) P = 0.709; ***(f) P = 0.0001), CHI3L1 ( ****(a) P < 0.0001; (ns) not significant (b) P = 0.1499; ****(c) P < 0.0001; ****(d) P < 0.0001; **(e) P = 0.0011; ****(f) P < 0.0001), ITGAX ( ***(a) P = 0.0004; (ns) not significant (b) P = 0.1892; ***(c) P = 0.0003; **(d) P = 0.004; (ns) not significant (e) P = 0.9899; **(f) P = 0.0028), SNHG15 ( ****(a) P < 0.0001; (ns) not significant (b) P = 0.7576; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P = 0.2091; ****(f) P < 0.0001), TFE3 ( ****(a) P < 0.0001; ****(b) P < 0.0001; ****(c) P < 0.0001; *(d) P = 0.0108; (ns) not significant (e) P = 0.1693; (ns) not significant (f) P = 0.2653) (one-way ANOVA followed by Tukey’s multiple comparison post-test). ( C ) Immunoblot analysis of protein lysates from ARPE19 cells infected with the indicated adenovirus for 30 h. Asterisk (*) indicates unspecific band. ( D ) Quantification of protein levels showing CD11c/GAPDH or YKL-40/GAPDH ratios expressed as fold change as shown in ( C ). Data are presented as mean ± SD of three independent experiments. (ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P = 0.9986; ****(d) P < 0.0001 (one-way ANOVA followed by Dunnett’s multiple comparison post-test). ( E ) Immunofluorescence confocal microscopy of ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc or Control for 30 h, showing the cellular distribution of YKL-40 (green). Scale bars: 10 μm. ( F ) Relative quantitative RT-PCR analysis of the mRNA expression of transcription regulator genes ( CITED1 and C/EBPα ) in ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc or Ad-Null for 30 h. Data are presented as mean ± SD of three independent experiments. CITED1 ( ***(a) P < 0.0001; (ns) not significant (b) P = 0.1888; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P > 0.9999; ****(f) P < 0.0001), C/EBPα ( ***(a) P < 0.0001; (ns) not significant (b) P = 0.1888; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P > 0.9999; ****(f) P < 0.0001) (one-way ANOVA followed by Tukey’s multiple comparison post-test). ( G ) Immunoblot analysis of protein lysates from ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc, or Ad-Null for 30 h. ( H ) Quantification of protein levels showing CITED1/GAPDH, LAMP1/GAPDH, C/EBPα-p42/GAPDH, C/EBPα-p30/GAPDH, and LC3-II/LC3-I ratios expressed as fold change as shown in ( G ). Data are presented as mean ± SD of three independent experiments. CITED1 ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ****(d) P < 0.0001), C/EBPα (p42) ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ***(d) P = 0.0002 ), LAMP1 ((ns) not significant (a) P = 0.956; *(b) P = 0.0127; **(c) P = 0.0023; **(d) P = 0.0044), C/EBPα (p30) ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ***(d) P = 0.0001), LC3 ((ns) not significant (a) P = 0.8551; ****(b) P < 0.0001; ***(c) P = 0.0009; ****(d) P < 0.0001) (one-way ANOVA followed by Dunnett’s multiple comparison post-test). .

Article Snippet: CITED1 Polyclonal antibody , Proteintech , Cat# 26999-1-AP, RRID:AB_2296838.

Techniques: Infection, Expressing, Recombinant, Quantitative RT-PCR, Control, Comparison, Western Blot, Immunofluorescence, Confocal Microscopy

Journal: PLoS ONE

Article Title: Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells

doi: 10.1371/journal.pone.0049452

Figure Lengend Snippet: ( A ) Expression profiles of the common brown fat-selective genes (Group A), classical brown fat-selective genes (Group B), and beige cell-selective genes (Group C) in human BAT from multiple adipose depots. The color scale shows the mRNA levels of the genes in green (low or no-expression)-black-red (high-expression) scheme. All genes are shown in the identical scale. WAT: white adipose tissue. M: smooth muscle. Each alphabet indicates the tissues from the same patient. ( B ) Correlation matrix of beige-selective and classical brown-selective gene expression. The color scale shows the Pearson correlation between each gene mRNA expression in blue (negative correlation, −1.0)-gray (no correlation, 0)-red (positive correlation, 1.0) scheme. ( C ) Strength of correlation with representative group A gene ( PRDM16 and PGC1a ) and classical-brown-selective genes or beige cell-selective genes. ** P <0.01 relative to representative group B gene. ( D ) Correlation between the mRNA expression of PRDM16 and PGCa1 (left), CITED1 (middle), and ZIC1 (right). These genes represent each gene class. Dotted lines denote density ellipse (95% confidence interval of plot). ( E ) Box-and-whisker plot (upper) and histogram (bottom) to graphically summarize the distribution of each gene expression levels. The lines extending from end of the box (quartile) are whiskers, which edge is the outermost data point(s) that fall within the distance defined by quartile ±1.5*(IQR). Values beyond the whiskers, denoted by a red point indicate outlier samples.

Article Snippet: They were subjected to citrate-based antigen retrieval using Antigen Retrieval Citra Solution (Biogenex) and incubated for 1 hour at room temperature with rabbit polyclonal antibodies against CITED1 at a dilution of 1∶500 (Abcam) or UCP1 at a dilution of 1∶1000 (Abcam) or IgG as negative control.

Techniques: Expressing, Gene Expression, Whisker Assay

Journal: PLoS ONE

Article Title: Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells

doi: 10.1371/journal.pone.0049452

Figure Lengend Snippet: ( A ) Immunohistochemistry of UCP1 (left) and CITED1 (right) in serial sections of WAT in mice that were treated with CL316243 at a dose of 1 mg/kg (upper panels) or saline control (lower panels) for 8 days . Scale bar, 50 µm. ( B ) Immunohistochemistry of UCP1 (left) and CITED1 (right) in serial sections of human BAT. Lower panels show IgG negative controls of immunohistochemistry for UCP1 (Cy3) and for CITED1 (FITC), respectively in human BAT. Scale bar, 50 µm.

Article Snippet: They were subjected to citrate-based antigen retrieval using Antigen Retrieval Citra Solution (Biogenex) and incubated for 1 hour at room temperature with rabbit polyclonal antibodies against CITED1 at a dilution of 1∶500 (Abcam) or UCP1 at a dilution of 1∶1000 (Abcam) or IgG as negative control.

Techniques: Immunohistochemistry, Saline, Control